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Expression pattern of oxidative stress and antioxidant defense-related genes in the aging Fischer 344/NHsd rat cochlea

  • Chiemi Tanaka

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • Oregon Hearing Research Center, Oregon Health and Science University, Portland, OR, USA
    • Corresponding Author InformationCorresponding author at: Oregon Hearing Research Center, Department of Otolaryngology, Mail Code NRC04, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA. Tel.: +1 503 494 2996; fax: +1 503 494 0951
    • ,
  • Donald E. Coling

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • ,
  • Senthilvelan Manohar

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • ,
  • Guang-Di Chen

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • ,
  • Bo Hua Hu

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • ,
  • Richard Salvi

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA
    • ,
  • Donald Henderson

      Affiliations

    • Center for Hearing and Deafness, State University of New York at Buffalo, Buffalo, NY, USA

Received 27 March 2011; received in revised form 13 December 2011; accepted 22 December 2011. published online 03 February 2012.
Corrected Proof

Abstract 

The biological mechanisms that give rise to age-related hearing loss (ARHL) are still poorly understood. However, there is growing recognition that oxidative stress may be an important factor. To address this issue, we measured the changes in the expression of cochlear oxidative stress and antioxidant defense-related genes in young (2 months old), middle-aged (12 months old), and old (21–25 months old) Fischer 344/NHsd (F344/NHsd) rats and compared gene expression changes with ARHL. A quantitative real-time reverse transcription polymerase chain reaction array revealed a significant age-related downregulation of only 1 gene, stearoyl-coenzyme A desaturase 1, and upregulation of 12 genes: 24-dehydrocholesterol reductase; aminoadipate-semialdehyde synthase; cytoglobin; dual oxidase 2; glutathione peroxidase 3; glutathione peroxidase 6; glutathione S-transferase, kappa 1; glutathione reductase; nicotinamide adenine dinucleotide phosphate (NAD(P)H) dehydrogenase, quinone 1; solute carrier Family 38, Member 5; thioredoxin interacting protein; and vimentin. Statistical analyses revealed significant correlations between gene expression and auditory function in 8 genes. Our results identified specific subsets of oxidative stress genes that appear to play an important role in ARHL in the Fischer 344/NHsd rat.

Keywords:  Age-related hearing loss , Presbycusis , Cochlea , Fischer 344 , Antioxidant , Reactive oxygen species , Oxidative stress , Rat , Genes , Scd1 , Dhcr24 , Aass , Cygb , Duox2 , Gpx3 , Gpx6 , Gstk1 , Gsr , Nqo1 , Slc38a5 , Txnip , Vim

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PII: S0197-4580(11)00572-0

doi:10.1016/j.neurobiolaging.2011.12.027

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