Association of AICD and Fe65 with Hirano bodies reduces transcriptional activation and initiation of apoptosis

Abstract 

Hirano bodies are cytoplasmic inclusions predominantly found in the central nervous system associated with various conditions including aging and Alzheimer's disease (AD). Since most studies of Hirano bodies have been performed in post-mortem samples, the physiological roles of Hirano bodies have not been investigated. Astrocytoma H4 cells were employed to test the hypothesis that Hirano bodies interact with and modulate signaling by the C-terminal fragment of amyloid-β precursor protein (AICD). We demonstrated by immunofluorescence and immunoprecipitation that model Hirano bodies accumulate AICD. Since stimulation of transcription by AICD is dependent on its interaction with the nuclear adaptor protein Fe65, we examined localization of Fe65, and employed a dual luciferase reporter assay to test the effects of Hirano bodies on AICD- and Fe65-dependent modulation of gene expression. We find that both AICD and Fe65 are co-localized in model Hirano bodies. Model Hirano bodies also down-regulate both AICD-dependent apoptosis and AICD- and Fe65-dependent transcriptional activity. Thus, association of AICD and Fe65 with Hirano bodies impedes their function in promoting apoptosis and modulating transcription.

Abbreviations: APP, amyloid-β precursor protein, AICD, the C-terminal fragment of amyloid-β precursor protein, APPx, all truncated forms of APP that include the C-terminal region, β-gal, β-galactosidase, PI, propidium iodide, MAP, microtubule-associated protein, LRP, Low density lipoprotein-related protein

Keywords: AICD, Amyloid precursor protein, Fe65, Hirano body, Actin filaments, Alzheimer's disease, Neurodegenerative disease, Apoptosis