« Previous
Next »
Neurobiology of Aging
Volume 31, Issue 4
, Pages 654-664
, April 2010
Inter-age variability of bona fide unvaried transcripts: Normalization of quantitative PCR data in ischemic stroke
References
- . Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64:5245–5250
- . Middle cerebral artery occlusion in the mouse by intraluminal suture coated with poly-L-lysine: neurological and histological validation. Brain Res. 1999;833:181–190
- . Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 2002;29:23–39
- . Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Technol. 2004;15:155–166
- . Rodent models of focal stroke: size, mechanism, and purpose. NeuroRx. 2005;2:396–409
- . The need for multiple time points in aging studies. Neurobiol. Aging. 2004;25:3–4
- . Normalization of gene expression measurements in tumor tissues: comparison of 13 endogenous control genes. Lab Invest. 2005;85:154–159
- . Genotype and age influence the effect of caloric intake on mortality in mice. FASEB J. 2003;17:690–692
- . Hypoxic regulation of endothelial glyceraldehyde-3-phosphate dehydrogenase. Am. J. Physiol. 1998;274:C347–C355
- . Identification and validation of endogenous reference genes for expression profiling of T helper cell differentiation by quantitative real-time RT-PCR. Anal. Biochem. 2001;299:63–70
- . The use of quantitative RT-PCR to measure mRNA expression in a rat model of focal ischemia – caspase-3 as a case study. Brain Res. Mol. Brain Res. 2000;75:143–149
- . Increased expression of glyceraldehyde-3-phosphate dehydrogenase in cultured astrocytes following exposure to manganese. Neurochem. Int. 1999;35:11–17
- . Real-time RT-PCR normalisation; strategies and considerations. Genes Immun. 2005;6:279–284
- . Adrenocorticotropin increases expression of c-fos and beta-actin genes in the rat adrenals. Endocrinology. 1990;127:1742–1747
- . Evidence that glyceraldehyde-3-phosphate dehydrogenase is involved in age-induced apoptosis in mature cerebellar neurons in culture. J. Neurochem. 1996;66:928–935
- . Experimental studies of ischemic brain edema 1 A new experimental model of cerebral embolism in ratsin which recirculation can be introduced in the ischemia area. Jpn. J. Stroke. 1986;8:1–8
- . Gene-expression profile of the ageing brain in mice. Nat. Genet. 2000;25:294–297
- . Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke. 1989;20:84–91
- . Central nervous system injury-induced immune deficiency syndrome. Nat. Rev. Neurosci. 2005;6:775–786
- . Gene expression studies in prostate cancer tissue: which reference gene should be selected for normalization?. J Mol Med. 2005;83:1014–1124
- . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29:e45
- . Heart disease and stroke statistics – 2007 update: a report from the American heart association statistics committee and stroke statistics subcommittee. Circulation. 2007;115:e69–171
- . Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol. 2000;132:365–386
- . Glyceraldehyde-3-phosphate dehydrogenase mRNA is a major interleukin 2-induced transcript in a cloned T-helper lymphocyte. Gene. 1990;91:185–191
- . Control selection for RNA quantitation. Biotechniques. 2000;29:332–337
- . Induction of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression in rat brain after focal ischemia/reperfusion. J. Cereb. Blood Flow Metab. 2002;22:280–288
- . Cost of stroke in Europe. Eur J Neurol. 2005;12(Suppl 1):78–84
- . Stroke incidence and prevalence in Europe: a review of available data. Eur J Neurol. 2006;13:581–598
- Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., Speleman, F., 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, RESEARCH0034.1–.11.
- . Variations in stroke incidence and survival in 3 areas of Europe. European Registries of Stroke (EROS) Collaboration. Stroke. 2000;31:2074–2079
- . Early changes of gene expression during cerulein supramaximal stimulation. Pancreas. 1999;19:45–50
- . Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem. Biophys. Res. Commun. 1999;259:523–526
PII: S0197-4580(08)00186-3
doi: 10.1016/j.neurobiolaging.2008.05.023
© 2008 Elsevier Inc. All rights reserved.
« Previous
Next »
Neurobiology of Aging
Volume 31, Issue 4
, Pages 654-664
, April 2010
