Neurobiology of Aging
Volume 28, Issue 4 , Pages 497-506, April 2007

Differential gene expression profiles in the hippocampus of senescence-accelerated mouse

  • Xiao-Rui Cheng

      Affiliations

    • Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China
    • ,
  • Wen-Xia Zhou

      Affiliations

    • Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China
    • Corresponding Author InformationCorresponding author. Tel.: +86 10 66931625; fax: +86 10 68211656.
    • ,
  • Yong-Xiang Zhang

      Affiliations

    • Beijing Institute of Pharmacology and Toxicology, Beijing 100850, PR China
    • ,
  • Dong-Sheng Zhou

      Affiliations

    • Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
    • ,
  • Rui-Fu Yang

      Affiliations

    • Beijing Institute of Microbiology and Epidemiology, Beijing 100071, PR China
    • ,
  • Ling-Feng Chen

      Affiliations

    • Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR China

Received 21 June 2005; received in revised form 27 January 2006; accepted 10 February 2006. published online 27 March 2006.

Abstract 

The senescence-accelerated mouse (SAM) is an animal model for studying senescence and age-associated disorders due to its inherited aging phenotype. The SAM/prone8 (SAMP8) is a useful animal model to investigate the fundamental mechanisms involved in age-related learning and memory deficits that may have relevance to age-associated AD, while SAM/resistant1 (SAMR1) shows normal. To identify genes rendering the cognitive deterioration with aging, the subtractive cDNA libraries containing 1924 clones with the positive ratio of 96.18% were generated and the microarray containing 3136 cDNA was prepared. The results of screening libraries by the microarray showed that of all 91 differentially expressed genes, 50 were over-expressed and 41 were low-expressed in SAMP8. Some of the identified genes were confirmed by the real time quantitative RT-PCR. These results indicated the profiles of gene expression in the hippocampus of SAMP8 and SAMR1 were significantly different, which may play important roles in the age-related cognitive deficit in SAMP8, suggesting those genes related to the cognitive deficient or pathology change in the brain of SAMP8 may be potential gene targets for Alzheimer's disease therapy.

Keywords: Senescence-accelerated mouse, Alzheimer's disease, cDNA microarray

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PII: S0197-4580(06)00050-9

doi:10.1016/j.neurobiolaging.2006.02.004

Neurobiology of Aging
Volume 28, Issue 4 , Pages 497-506, April 2007

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